Anne E. Bernhard Ph.D. Curriculum Vitae

Ph.D. Oregon State University, Corvallis, Oregon (Microbiology), July 2000; Thesis: Molecular markers from fecal anaerobes to identify sources of fecal pollution in coastal waters; Major Adviser, Katharine G. Field

M.S. Western Washington University, Bellingham, Washington (Environmental Science-Marine and Estuarine Science option), August 1993; Thesis: Nutrient limitation of phytoplankton in Padilla Bay, WA; Major Adviser, Emily R. Peele

B.S. Texas A&M University, College Station, Texas (Biology), May 1987, Study Abroad, University of Essex, Colchester, England, Fall 1985

George and Carol Milne Assistant Professor in Life Science, 2006-present, Department of Biology, Connecticut College, New London, Connecticut

Assistant Professor, 2004-2006, Department of Biology, Connecticut College, New London, CT

NSF Postdoctoral Research Fellow, Department of Civil and Environmental Engineering, University of Washington, Seattle, Washington, 2002-2004

Undergraduate Research Faculty Mentor, Engr100H, Introduction to Engineering Design University of Washington, College of Engineering, Seattle, Washington, 2002-2003

Co-Instructor, CEE550, Microbial Process Fundamentals, University of Washington, Dept. of Civil & Environmental Engineering, Seattle, Washington, Fall 2001 and 2002

Guest Lecturer, CEE564, Aquatic Chemistry Lab, University of Washington, Seattle, Washington, Spring 2002 and Winter 2003

Postdoctoral Research Associate, Department of Civil and Environmental Engineering, University of Washington, Seattle, Washington, 2000-2002

Graduate Research Assistant, Department of Microbiology, Oregon State University, Corvallis, Oregon, 1998-2000

Graduate Teaching Assistant, Department of Microbiology, Oregon State University, Corvallis, Oregon, 1996-1998

Instructor, Huxley College of Environmental Studies, Western Washington University, Bellingham, Washington, 1994-1995

Scientific Technician, Washington State Department of Fish and Wildlife, Olympia, Washington, 1994

Research Assistant, University of Alaska-Fairbanks, Juneau School of Fisheries and Ocean Sciences, Juneau, Alaska, 1993-1994

Graduate Teaching Assistant, Huxley College of Environmental Studies, Western Washington University, Bellingham, Washington, 1991-1993

Research Assistant II, (1988-1991 and 1995-1996) University of Texas Health Science Center, Department of Biochemistry and Molecular Biology, Houston, Texas

High School Biology and Chemistry Teacher, Incarnate Word Academy, Houston, Texas, 1987-1988

Bernhard AE, Tucker J, Giblin AE, Stahl DA. (2007) Functionally distinct communities of ammonia oxidizing bacteria along an estuarine salinity gradient. Environmental Microbiology 9:1439-1447.

(Koenneke M, Bernhard AE, de la Torre JR)†,Walker CM, Waterbury JB, Stahl DA. (2005) Isolation of an autotrophic ammonia-oxidizing marine archaeon. Nature 437:543-546.
†authors contributed equally

Bernhard AE, Donn T*, Giblin AE, Stahl DA. (2005) Loss of diversity of ammonia-oxidizing bacteria correlates with increasing salinity in an estuary system. Environmental Microbiology 7: 1289-1297.

Dick LK, Bernhard AE, Brodeur TJ, Santo Domingo JW, Simpson JM, Walters SP, and Field KG. (2005). Host Distributions of Uncultivated Fecal Bacteroidales Reveal Genetic Markers for Fecal Source Identification. Applied and Environmental Microbiology 71:3184-3191.

Bernhard AE, Colbert D, McManus J, Field KG. (2005) Microbial community dynamics measured by 16S ribosomal DNA profiles along a salinity gradient in a shallow Northwest estuary. FEMS Microbiology Ecology 52:115-128.

Nielsen JL, Schramm A, Bernhard AE, van den Engh GJ, Stahl DA. (2004) Flow cytometry-assisted clone sorting after fluorescence in situ hybridization (FACS-FISH) of 16S rRNA gene clone libraries. Applied and Environmental Microbiology 70: 7550-7554.

El Fantroussi S, Urakawa H, Bernhard AE, Kelly JJ, Noble PA, Smidt H, Yershov GM, Stahl DA. (2003) Direct profiling of environmental microbial populations by thermal dissociation analysis of native rRNAs hybridized to oligonucleotide microarrays. Applied and Environmental Microbiology 69: 2377-82.

Bernhard AE, Goyard T*, Simonich MT, Field KG. (2003) Application of a rapid method for identifying fecal pollution sources in a multi-use estuary. Water Research 37:909-913.

Field KG., Bernhard AE, (2001) Molecular detection of fecal Bacteroides and source indicators for fecal pollution in water. In Warwick, J. J. (ed.), Water quality monitoring and modeling, American Water Resources Association, Spring Specialty Conference, San Antonio, TX.

Bernhard AE, Field KG. (2000) A PCR assay to discriminate human and ruminant feces on the basis of host-specific Bacteroides/Prevotella 16S rDNA. Applied and Environmental Microbiology 66: 4571-4574.

Bernhard AE, Field KG. (2000) Identification of nonpoint source pollution in coastal waters by using 16S ribosomal DNA genetic markers from fecal anaerobes. Applied and Environmental Microbiology 66: 1587-1594.

Bernhard AE, Peele ER. (1997) Nitrogen limitation of phytoplankton in a shallow embayment in Northern Puget Sound. Estuaries 20:759-769.

*undergraduate researcher

Connecticut Department of Environmental Protection, Long Island Sound Funds Program
Feb 2006-Feb 2007, $23,440, Impacts of salt marsh restoration on microbial community structure and diversity

NSF Research Starter Grant, Aug 2005-Aug 2006, $42,500
Population dynamics of ammonia-oxidizing bacteria in estuarine sediments, Principal Investigator

NSF Postdoctoral Research Fellowship in Microbial Biology, 2002-2004, $100,000.
amoA mRNA as an indicator for activity of ammonia-oxidizing bacteria in estuarine sediments

Washington State Department of Ecology
Seasonal study of phytoplankton growth limitation, June 1992- July 1993, $3600

Marine Biological Laboratory, Woods Hole, MA, Feb 2006

University of Massachusetts, Lowell Campus, Environmental Science Seminar, Lowell, MA, Sept 2005

Trinity College, Dept. of Biology Seminar, Hartford, CT, Oct 2005

Connecticut College, Dept. of Biology Seminar, New London, CT, Jan 2004

Texas State University, Dept. of Biology Seminar, San Marcos, TX, Feb 2004

University of Washington, Dept. of Civil and Environmental Engineering Seminar, Seattle, WA, Sept 2003

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